1. Trihydroxymethyl aminomethane bakeng sa tataiso ea tlhahiso ea indasteri

Ka lebaka la sebopeho sa eona se bonolo, athomo ea carbon e bohareng ke sebopeho sa tetrahedral, se nang le li-hydroxymethyl tse tharo le sehlopha sa amino, se ka khonang ho etsa mefuta e fapaneng ea phetoho le karabelo ea REDOX, ke mofuta oa lisebelisoa tse tala tsa boleng bo phahameng ba lik'hemik'hale. E 'ngoe ea mekhoa ea ho kopanya TAPS ea buffer e entsoe ho sebelisoa Tris le 1, 3-propanolone ka joala. Hangata, lisebelisoa tse tala tsa TRIS tse sebelisetsoang motsoako oa indasteri ha li hloke bohloeki bo phahameng ba reagent. Ho hatisoa ka bongata bo boholo, kahoo ho na le litšenyehelo tse ngata ho fumana moetsi oa thepa e tala ea nang le matla a maholo.

2. Trimethylol amino methane bakeng sa tataiso ea ho lemoha lik'hemik'hale

Hangata Tris e sebelisoa e le tšireletso ea likokoana-hloko ho boloka pH ea karabelo ea lik'hemik'hale. Ka lebaka la ho fokola ha alkalinity, Tris hangata e sebelisoa hammoho le li-acids. Haholo-holo e sebelisetsoa nucleic acid, protheine dissolution buffer, protheine imprinting buffer, DNA imprinting test buffer, DNA agarose gel electrophoresis buffer, joalo-joalo e batlang e phahame, kahoo ho reka ho hloka ho khetha moetsi ka lilemo tse ngata tsa lipatlisiso le nts'etsopele le phihlelo ea tlhahiso, bohloeki bo phahameng, litšila tse fokolang ho netefatsa ho nepahala ha liteko tsa biochemical. Tekanyo ea mocheso oa tris buffer, ho bonolo ho monya carbon dioxide moeeng, tlhophiso ea buffer e tlameha ho koaheloa e tiisitsoe ka thata.

3. Trimethylol aminomethane bakeng sa lipatlisiso tsa meriana le nts'etsopele

Li-hydroxymethyl aminomethane tse ling tse tharo, e leng trometamol, kaha ha li na sodium amino buffer base, ka marong a 'mele a itšoarang ka H2CO3, a fokotsa H2CO3, a hlahisa HCO32 - hape ka nako e ts'oanang, a ka monya li-ion tsa hydrogen le acidemia e nepahetseng, phello ea eona e matla le ho feta. , 'me e ka ka lera sele, hangata sebelisoa ka a hlobaetsang le metabolic le ho hema acidemia; E le lithethefatsi tsa alkaline, tse sebelisetsoang ho lokisa acidosis ntle le ho baka ho boloka carbon dioxide. Hobane e sebelisoa bakeng sa meriana, tlhahiso eohle ea mohloli oa thepa e tala ea TRIS, tsela ea ts'ebetso ea tlhahiso, bohloeki ba sehlahisoa, litaba tsa ion tse sa hloekang ke litlhokahalo tse thata, theko ea lihlahisoa tsa mofuta ona e tlameha ho ba thata haholo le ho ba hlokolosi, e tlameha ho feta liteko tse thata.

4. Trimethylaminomethane e sebelisoa mokelikeli oa polokelo ea kokoana-hloko

Tharollong ea paballo ea kokoana-hloko, Tris e ka qala ka ho boloka boleng ba pH ba sampole, e sebetsa e le tšireletso ea likokoana-hloko, 'me mofuta oa buffer oa pH oa Tris ke: 7.0 ho isa ho 9.0, bokhoni ba ho boloka ho qhekella ha sampole kamora ho lokolloa ha botsitso ba nucleic acid, ho qoba ho senyeha ha nucleic acid, le ho ntlafatsa ho tsepamisa mohopolo le ho hloeka ha nucleic acid, ho netefatsa ho nepahala ha ts'ebetso e latelang e kang tlhahlobo ea tlhahlobo ea nucleic acid, ka lebaka la nako e khethehileng ea seoa, litlhoko tsa ho fumanoa ha nucleic acid, Tris. theko ea bongata e tla ba kholoanyane, kahoo fana ka maikutlo a ho reka lihlahisoa tse joalo li tlameha ho qala ho reka palo e nyane ea sehlahisoa pele u reka teko e kholo e netefalitsoeng hore ha ho na bothata, hape e tlameha ho ba le botekgeniki kamora thekiso ho fana ka ts'ehetso ea botekgeniki ea litsebi.